Cartpt^{tm1.1(cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Cartpt^{tm1.1(cre)Hze}
• Name: CART prepropeptide; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5704439
• Synonyms: Cart-IRES2-Cre-D
• Gene: Cartpt Location: Chr13:100034991-100037191 bp, - strand Genetic Position: Chr13, 52.9 cM, cytoband D1
• Alliance: Cartpt^{tm1.1(cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146821
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Cartpt^{tm1.1(cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cartpt (MGI:1351330)

• Mutation details: The targeting vector contains, from 5' to 3', a partial Cartpt sequence spanning exon 1 through intron 2, an FRT3 site within Cartpt intron 2, a partial Cartpt exon 3 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an FRT5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 late polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). (J:146821)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cartpt (mouse) Summary of all recombinase alleles driven by Cartpt. Comparative matrix view of recombinase activities Alliance mouse Cartpt gene page
User Notes:	Additional information submitted by the research community Additional information submitted by the research community The donating investigator reports Cre recombinase expression and activity largely recapitulates the endogenous Cartpt gene's unique expression pattern in areas such as cortex, cerebral nuclei, hypothalamus and midbrain. The donating investigator did not examine cre expression in tissues other than brain.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cartpt Mutation: 15 strains or lines available

References

• Original: J:146821 Allen Institute for Brain Science, Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS). MGI Direct Data Submission. 2009-04-06
• All: 16 reference(s)