Trit1^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Trit1^{tm1.1(KOMP)Vlcg}
• Name: tRNA isopentenyltransferase 1; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5704516
• Synonyms: Trit1^-
• Gene: Trit1 Location: Chr4:122910390-122948742 bp, + strand Genetic Position: Chr4, 57.36 cM
• Alliance: Trit1^{tm1.1(KOMP)Vlcg} page
• IMPC: Trit1 gene page

Trit1^{tm1.1(KOMP)Vlcg}/Trit1^{tm1.1(KOMP)Vlcg} mice exhibit embryonic lethality, with fewer than expected number of embryos at E7.5 and no embryos at E9.5. Though an egg cyclinder is formed, no morphological node or other hallmarks typical of E7.5 are present.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: 13142A-H7
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: Cre-mediated excision of the parental Trit1^{tm1(KOMP)Vlcg} allele resulted in the removal of the neomycin selection cassette and critical exon(s) leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:204739)

Expression

In Mice Carrying this Mutation:	37 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trit1 Mutation: 44 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 5 reference(s)