Brpf1^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Brpf1^{tm1b(EUCOMM)Wtsi}
• Name: bromodomain and PHD finger containing, 1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5705128
• Synonyms: Brpf1^delta
• Gene: Brpf1 Location: Chr6:113284098-113301821 bp, + strand Genetic Position: Chr6, 52.75 cM, cytoband E3
• Alliance: Brpf1^{tm1b(EUCOMM)Wtsi} page
• IMPC: Brpf1 gene page

Mutation origin

• Mutant Cell Lines: EPD0069_1_G04, EPD0069_1_G06
• Germline Transmission: Earliest citation of germline transmission: J:222304
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 113293068 of Chromosome 6 upstream of the critical exons 4 through 6 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons 4-6 at position 113294936. The critical exons 4 through 6 are thus flanked by loxP sites. Cre-mediated recombination removed exons 4-6 and left the FRT flanked lacZ/neomycin sequence followed by one loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:222304)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brpf1 Mutation: 37 strains or lines available

Notes

Cell line EPD0069_1_G06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:222304

References

• Original: J:222304 You L, et al., Expression atlas of the multivalent epigenetic regulator Brpf1 and its requirement for survival of mouse embryos. Epigenetics. 2014 Jun;9(6):860-72
• All: 1 reference(s)