Brpf1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Brpf1^{tm1c(EUCOMM)Wtsi}
• Name: bromodomain and PHD finger containing, 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5705131
• Synonyms: Brpf1^f
• Gene: Brpf1 Location: Chr6:113284098-113301821 bp, + strand Genetic Position: Chr6, 52.75 cM, cytoband E3
• Alliance: Brpf1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Brpf1 gene page

Mutation origin

• Mutant Cell Lines: EPD0069_1_G04, EPD0069_1_G06
• Germline Transmission: Earliest citation of germline transmission: J:222304
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 113293068 of Chromosome 6 upstream of the critical exons 4 through 6 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons 4-6 at position 113294936. The critical exons 4 through 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:222304)

Disease models

Expression

In Mice Carrying this Mutation:	96 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brpf1 Mutation: 37 strains or lines available

Notes

Cell line EPD0069_1_G06 was successfully used to make chimeric mice. Germline transmission was accomplished. J:222304

References

• Original: J:222304 You L, et al., Expression atlas of the multivalent epigenetic regulator Brpf1 and its requirement for survival of mouse embryos. Epigenetics. 2014 Jun;9(6):860-72
• All: 7 reference(s)