Col1a1^{tm1(tetO-Ccnb1)Jvd}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-Ccnb1)Jvd}
• Name: collagen, type I, alpha 1; targeted mutation 1, Jan M A van Deursen
• MGI ID: MGI:5705289
• Synonyms: Ccnb1-HA, Ccnb1^T11, Ccnb1^T7
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-Ccnb1)Jvd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219399
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-Ccnb1)Jvd} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ccnb1 (MGI:88302)

• Col1a1^{tm1(tetO-Ccnb1)Jvd} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: RMCE on KH2 cells replaced the FRT-flanked neo cassette with a cassette that contains a splice acceptor, polyadenylation sequence and the tetracycline promoter driving inducible expression of an HA-tagged cDNA for mouse Ccnb1 followed by a polyadenylation sequence and hygromycin resistance cassette with an FRT site. (J:219399)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:219399 Nam HJ, et al., Cyclin B2 and p53 control proper timing of centrosome separation. Nat Cell Biol. 2014 Jun;16(6):538-49
• All: 1 reference(s)