Scube1^tm2.1Rbya
Targeted Allele Detail

Summary

• Symbol: Scube1^tm2.1Rbya
• Name: signal peptide, CUB domain, EGF-like 1; targeted mutation 2.1, Ruey-Bing Yang
• MGI ID: MGI:5705524
• Synonyms: Scube1^delta
• Gene: Scube1 Location: Chr15:83489200-83609222 bp, - strand Genetic Position: Chr15, 39.4 cM
• Alliance: Scube1^tm2.1Rbya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:223899
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 15. A floxed neomycin resistance cassette was inserted downstream of exon 18. Cre-mediated recombination removed exons 15 through 18 (encoding part of the spacer region and the complete 3 cysteine-rich (CR) repeat motifs) and the selection cassette. RT-PCR analysis followed by direct sequencing showed that while exons 15-18 were successfully deleted, the COOH-terminal CUB domain was transcribed via in-frame coding in homozygous mutant platelets. Immunostaining using anti-CR specific antibody confirmed a complete absence of the 3 CR repeats but conservation of the CUB domain in mutant platelets. The soluble form of SCUBE1 protein was undetectable in mutant mice. (J:223899)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scube1 Mutation: 48 strains or lines available

References

• Original: J:223899 Wu MY, et al., Inhibition of the plasma SCUBE1, a novel platelet adhesive protein, protects mice against thrombosis. Arterioscler Thromb Vasc Biol. 2014 Jul;34(7):1390-8
• All: 1 reference(s)