Dmxl2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dmxl2^{tm1c(EUCOMM)Wtsi}
• Name: Dmx-like 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5705577
• Synonyms: Dmxl2^lox
• Gene: Dmxl2 Location: Chr9:54272442-54408910 bp, - strand Genetic Position: Chr9, 29.58 cM, cytoband C
• Alliance: Dmxl2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Dmxl2 gene page

Fewer GnRH-ir neurons in the hypothalamus of Dmxl2^{tm1c(EUCOMM)Wtsi}/Dmxl2⁺ Tg(Nes-cre)1Atp/0 male mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: EPD0431_3_E07
• Germline Transmission: Earliest citation of germline transmission: J:217623
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 54367995 of Chromosome 9 upstream of the critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 7 at position 54368954. Flp-mediated recombination removed the lacZ sequence and neo cassette and left exon 7 floxed. (J:217623)

Generation of the Dmxl2^tm1a(EUCOMM)Wtsi and Dmxl2^tm1c(EUCOMM)Wtsi alleles

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dmxl2 Mutation: 122 strains or lines available

References

• Original: J:217623 Tata B, et al., Haploinsufficiency of Dmxl2, encoding a synaptic protein, causes infertility associated with a loss of GnRH neurons in mouse. PLoS Biol. 2014 Sep;12(9):e1001952
• All: 2 reference(s)