Ngly1^tm1.1Tasuz
Targeted Allele Detail

Summary

• Symbol: Ngly1^tm1.1Tasuz
• Name: N-glycanase 1; targeted mutation 1.1, Tadashi Suzuki
• MGI ID: MGI:5705596
• Gene: Ngly1 Location: Chr14:6157837-6220449 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A2
• Alliance: Ngly1^tm1.1Tasuz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217663
• Parent Cell Line: KY1.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 11 and an FRT flanked neo cassette and loxP site were inserted downstream of exon12 via homologous recombination. Cre mediated recombination removed exons 11 and 12 and the neo cassette. (J:217663)

Generation of the Ngly1^tm1Tasuz and Ngly1^tm1.1Tasuz alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ngly1 Mutation: 39 strains or lines available

References

• Original: J:217663 Huang C, et al., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc Natl Acad Sci U S A. 2015 Feb 3;112(5):1398-403
• All: 6 reference(s)