Gt(ROSA)26Sor^{em1(CAG-cas9*,-EGFP)Rsky}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-cas9*,-EGFP)Rsky}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Klaus Rajewsky
• MGI ID: MGI:5705809
• Synonyms: Rosa26^LSL-Cas9
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-cas9*,-EGFP)Rsky} page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Endonuclease, Reporter)
• Mutation: Insertion
• Mutation details: The template construct used for for homology-directed repair (HDR) was designed with (from 5' to 3') a 5' homology arm, splice acceptor, polyA elements, a ubiquitously expressed CAG promoter, two copies of the bovine growth hormone poly(A) signal, loxP-flanked Neo/Stop cassette, a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) linked to an IRES-GFP (internal ribosome entry site and Green Fluorescent Protein) reporter, and a 3' homology arm. The allele was generated by injecting the template construct, sgRosa26-1 guide RNA, and cas9 mRNA (Cas9-162A, containing plasmid coded polyadenine (polyA) tail). (J:229086)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:229086 Chu VT, et al., Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes. BMC Biotechnol. 2016;16(1):4
• All: 9 reference(s)