Tg(Col1a1-TK*)#Rowe
Transgene Detail

Summary

• Symbol: Tg(Col1a1-TK*)#Rowe
• Name: transgene insertion, David W Rowe
• MGI ID: MGI:5706722
• Synonyms: Col2.3deltatk
• Transgene: Tg(Col1a1-TK*)#Rowe Location: unknown
• Alliance: Tg(Col1a1-TK*)#Rowe page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A 2.3-kb fragment of rat alpha1 type I collagen promoter drives the expression of a truncated form of the herpes simplex virus thymidine kinase gene. Northern blot analysis confirmed transcript in long bones, calvaria and tail and immunostaining confirmed protein expression localized to cells lining the trabeculae and the endosteal surfaces, with lower levels in tendon. Osteoblast cell ablation is dependent on the administration of an antiviral prodrug such as ganciclovir. Two lines were generated (98-117 and 98-200), however, this record represents pooled data or data where the line number is not specified. (J:112520)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Phenotypic Similarity to Human Syndrome: Adynamic Bone Disease J:217781.

References

• Original: J:112520 Visnjic D, et al., Conditional ablation of the osteoblast lineage in Col2.3deltatk transgenic mice. J Bone Miner Res. 2001 Dec;16(12):2222-31
• All: 3 reference(s)