Col1a1^{tm1(tetO-RNAi:Ptgs2,GFP)Hahe}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-RNAi:Ptgs2,GFP)Hahe}
• Name: collagen, type I, alpha 1; targeted mutation 1, Harvey R Herschman
• MGI ID: MGI:5706736
• Synonyms: shCox-2, TG-Cox2.2058
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-RNAi:Ptgs2,GFP)Hahe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218934
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Knockdown, Reporter)
• Mutation: Insertion
• Col1a1^{tm1(tetO-RNAi:Ptgs2,GFP)Hahe} involves 1 genes/genome features (Ptgs2) View all
• Mutation details: RMCE on KH2 cells inserted into the 3' UTR the tetracycline promoter driving inducible expression of a short hairpin sequence targeting Ptgs2 (shRNA 2058), a puromycin resistance cassette, an IRES sequence, and a GFP reporter gene. (J:218934)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:218934 Zaiss AK, et al., Reversible suppression of cyclooxygenase 2 (COX-2) expression in vivo by inducible RNA interference. PLoS One. 2014;9(7):e101263
• All: 1 reference(s)