Plxnb2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Plxnb2^{tm1c(EUCOMM)Wtsi}
• Name: plexin B2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5706788
• Synonyms: Plexin-B2^fl
• Gene: Plxnb2 Location: Chr15:89039752-89064960 bp, - strand Genetic Position: Chr15, 44.68 cM, cytoband E3
• Alliance: Plxnb2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Plxnb2 gene page

Mutation origin

• Mutant Cell Line: EPD0051_2_D09
• Germline Transmission: Earliest citation of germline transmission: J:228347
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_st1
• Mutation details: The L1L2_st1 cassette was inserted at position 89047954 of Chromosome 15 upstream of the critical exons 8-10 (coding exons 7-9, Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 8-10 at position 89049222. The critical exons 8-10 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:228347)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plxnb2 Mutation: 88 strains or lines available

References

• Original: J:228347 Daviaud N, et al., Impaired cortical neurogenesis in plexin-B1 and -B2 double deletion mutant. Dev Neurobiol. 2016 Aug;76(8):882-99
• All: 5 reference(s)