Dpn1^PWK/PhJ
QTL Variant Detail

Summary

• QTL variant: Dpn1^PWK/PhJ
• Name: Der p 1-induced pulmonary neutrophils; PWK/PhJ
• MGI ID: MGI:5707070
• QTL: Dpn1 Location: unknown Genetic Position: Chr2, Syntenic

Variant origin

• Strain of Specimen: PWK/PhJ

Variant description

• Allele Type: QTL
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:217088

QTL Reference Notes

The Collaborative Cross (CC) is a large (~1,000 line) panel of recombinant inbred (RI) mouse strains being developed through a community effort (Churchill et al. 2004). The CC combines the genomes of eight genetically diverse founder strains - A/J, C57BL/6J, 129S1/SvImJ, NOD/ShiLtJ, NZO/HlLtJ, CAST/EiJ, PWK/PhJ, and WSB/EiJ - to capture nearly 90% of the known variation present in laboratory mice. CC strains are derived using a unique funnel breeding scheme. Once inbred, the RI CC lines can be used to generate thousands of potential 'outbred' but completely reproducible genomes through the generation of recombinant inbred crosses (RIX). The designation 'PreCC' is used to describe a mapping population of CC mice that is still at incipient stages of inbreeding. CTC (2004), Churchill, G. A., et al.. The Collaborative Cross, a community resource for the genetic analysis of complex traits. Nat Genet. 36, 1133-7.

Linkage analysis was performed on 151 male PreCC# mice using ~181,000 markers to identify QTL associated with airway polymorphonuclear neutrophil (PMN) recruitment. Mice were obtained from Oak Ridge National Laboratory (Oak Ridge, TN). Each mouse was from a distinct CC line that had undergone 514 generations of inbreeding.

QTL Dpn1 maps to 79.8 - 98 Mb on Chromosome 2 (Build 37) with a peak LOD score of 7.54 in linkage with polymorphonuclear neutrophil (PMN) recruitment. PWK/PhJ and CAST/EiJ alleles contribute to the phenotype at the Dpn1 locus. Zfp30 was identified as the underlying candidate gene.

References

• Original: J:217088 Rutledge H, et al., Genetic regulation of Zfp30, CXCL1, and neutrophilic inflammation in murine lung. Genetics. 2014 Oct;198(2):735-45
• All: 1 reference(s)