Crh^{tm2(icre)Ksak}
Targeted Allele Detail

Summary

• Symbol: Crh^{tm2(icre)Ksak}
• Name: corticotropin releasing hormone; targeted mutation 2, Kenji Sakimura
• MGI ID: MGI:5707348
• Synonyms: CRF-iCre, Crh^{tm2(icre)Itoi}
• Gene: Crh Location: Chr3:19747565-19749560 bp, - strand Genetic Position: Chr3, 5.75 cM
• Alliance: Crh^{tm2(icre)Ksak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218341
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Crh^{tm2(icre)Ksak} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Crh (MGI:88496)

• Mutation details: A codon-optimized cre (icre) recombinase gene and neomycin resistance cassette were inserted downstream of the translation initiation site in exon 2. (J:218341)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Crh (mouse) Summary of all recombinase alleles driven by Crh. Comparative matrix view of recombinase activities Alliance mouse Crh gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crh Mutation: 42 strains or lines available

References

• Original: J:218341 Itoi K, et al., Visualization of corticotropin-releasing factor neurons by fluorescent proteins in the mouse brain and characterization of labeled neurons in the paraventricular nucleus of the hypothalamus. Endocrinology. 2014 Oct;155(10):4054-60
• All: 6 reference(s)