Tle4^tm1.2Dasw
Targeted Allele Detail

Summary

• Symbol: Tle4^tm1.2Dasw
• Name: transducin-like enhancer of split 4; targeted mutation 1.2, David A Sweetser
• MGI ID: MGI:5707932
• Synonyms: Tle4Delta
• Gene: Tle4 Location: Chr19:14425514-14575415 bp, - strand Genetic Position: Chr19, 9.11 cM
• Alliance: Tle4^tm1.2Dasw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219243
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. A floxed neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 2, which encodes a lysine zipper motif, and results in a truncated protein. Western blot analysis confirmed the absence of protein expression in brain and lung. (J:219243)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tle4 Mutation: 57 strains or lines available

References

• Original: J:219243 Wheat JC, et al., The corepressor Tle4 is a novel regulator of murine hematopoiesis and bone development. PLoS One. 2014;9(8):e105557
• All: 3 reference(s)