Samd8^tm2Kwi
Targeted Allele Detail

Summary

• Symbol: Samd8^tm2Kwi
• Name: sterile alpha motif domain containing 8; targeted mutation 2, Klaus Willecke
• MGI ID: MGI:5707958
• Synonyms: SMSrflox
• Gene: Samd8 Location: Chr14:21800599-21848794 bp, + strand Genetic Position: Chr14, 11.86 cM, cytoband B
• Alliance: Samd8^tm2Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228568
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The gene has been modified by insertion of a loxP site upstream of exon 5, a frt-flanked SV40-neo cassette followed by a second loxP site downstream of the 3' UTR, and a second copy of exon 5 followed in-frame by the EGFP coding sequence. Cre recombinase-mediated excision of the endogenous exon 5 through the 3' untranslated region will result in splicing of exon 4 to the downstream copy of exon 5, leading to production of a SAMD8/EGFP fusion protein lacking the catalytic domain encoded by exon 6. (J:228568)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Samd8 Mutation: 31 strains or lines available

References

• Original: J:228568 Bickert A, et al., Functional characterization of enzymes catalyzing ceramide phosphoethanolamine biosynthesis in mice. J Lipid Res. 2015 Apr;56(4):821-35
• All: 1 reference(s)