Zfyve26^tm1.1Cahb
Targeted Allele Detail

Summary

• Symbol: Zfyve26^tm1.1Cahb
• Name: zinc finger, FYVE domain containing 26; targeted mutation 1.1, Christian A Hubner
• MGI ID: MGI:5707975
• Gene: Zfyve26 Location: Chr12:79279120-79343078 bp, - strand Genetic Position: Chr12, 35.51 cM
• Alliance: Zfyve26^tm1.1Cahb page

Abnormal gait of a Zfyve26^tm1.1Cahb/Zfyve26^tm1.1Cahb mouse walking on a beam

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:223127
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A neomycin cassette (Neo) flanked by frt sites and a loxP-site was inserted into intron 15. A second loxP-site together with a BamHI site was introduced into intron 14. Cre-mediated recombination removed exon 15 and the selection cassette. Northern blot analysis of total brain RNA from control and homozygous mutant animals showed that no expressed transcript is detectable. Western blot analysis with affinity purified antibodies against the N- terminus or the C-terminus of the encoded protein detected a polypeptide in brain extracts from wild-type but not from homozygous mutant mice. (J:223127)

Generation of the Zfyve26^tm1.1Cahb allele

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfyve26 Mutation: 106 strains or lines available

References

• Original: J:223127 Khundadze M, et al., A hereditary spastic paraplegia mouse model supports a role of ZFYVE26/SPASTIZIN for the endolysosomal system. PLoS Genet. 2013;9(12):e1003988
• All: 2 reference(s)