Abcb10^tm1.2Tafu
Targeted Allele Detail

Summary

• Symbol: Abcb10^tm1.2Tafu
• Name: ATP-binding cassette, sub-family B member 10; targeted mutation 1.2, Tatsuhiko Furukawa
• MGI ID: MGI:5708657
• Gene: Abcb10 Location: Chr8:124679198-124709861 bp, - strand Genetic Position: Chr8, 72.31 cM
• Alliance: Abcb10^tm1.2Tafu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:223428
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cre-mediated recombination removed the loxP flanked exon 3, causing a frameshift mutation at the splice junction site across exons 2 and 4. This results in disruption of 3 transmembrane motifs and an ATP-binding domain that is encoded by the downstream region of exon 3. Western blot analysis confirmed absence of protein in homozygotes. (J:223428)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Abcb10 Mutation: 42 strains or lines available

References

• Original: J:223428 Yamamoto M, et al., Abcb10 role in heme biosynthesis in vivo: Abcb10 knockout in mice causes anemia with protoporphyrin IX and iron accumulation. Mol Cell Biol. 2014 Mar;34(6):1077-84
• All: 1 reference(s)