Zfp809^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zfp809^{tm1c(KOMP)Wtsi}
• Name: zinc finger protein 809; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5749229
• Synonyms: Zfp809^FL
• Gene: Zfp809 Location: Chr9:22137010-22154650 bp, + strand Genetic Position: Chr9, 8.65 cM
• Alliance: Zfp809^{tm1c(KOMP)Wtsi} page
• IMPC: Zfp809 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219938
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: upstream of the exons 4 and 5 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 5 at position 22239485. Exons 4 and 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:173534, J:219938)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp809 Mutation: 68 strains or lines available

References

• Original: J:219938 Wolf G, et al., The KRAB zinc finger protein ZFP809 is required to initiate epigenetic silencing of endogenous retroviruses. Genes Dev. 2015 Mar 1;29(5):538-54
• All: 2 reference(s)