Tg(CAG-en/Hoxb5,-EGFP)#Vchl
Transgene Detail

Summary

• Symbol: Tg(CAG-en/Hoxb5,-EGFP)#Vchl
• Name: transgene insertion, Vincent Chi Hang Lui
• MGI ID: MGI:5749814
• Synonyms: enb5
• Transgene: Tg(CAG-en/Hoxb5,-EGFP)#Vchl Location: unknown
• Alliance: Tg(CAG-en/Hoxb5,-EGFP)#Vchl page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CAG-en/Hoxb5,-EGFP)#Vchl expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hoxb5 (MGI:96186)	The Drosophila engrailed repressor domain replaces the transcription activation domain of mouse Hoxb5

• Mutation details: The transgene contains a CAG promoter, floxed chloramphenicol acetyl transferase gene, a fusion gene in which the Drosophila engrailed repressor domain replaces the transcription activation domain of mouse Hoxb5, an IRES sequence and an EGFP reporter gene. Cre-mediated recombination is required to achieve expression of the fusion gene. Two lines (A2 and E4) achieved germline transmission. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:136122)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Phenotypic Similarity to Human Syndrome: Hirschsprung disease (J:136122)

References

• Original: J:136122 Lui VC, et al., Perturbation of hoxb5 signaling in vagal neural crests down-regulates ret leading to intestinal hypoganglionosis in mice. Gastroenterology. 2008 Apr;134(4):1104-15
• All: 3 reference(s)