Klf2^tm1Hhn
Targeted Allele Detail

Summary

• Symbol: Klf2^tm1Hhn
• Name: Kruppel-like transcription factor 2 (lung); targeted mutation 1, Huck Hui Ng
• MGI ID: MGI:5750084
• Synonyms: Klf2^Lac-NeoZ
• Gene: Klf2 Location: Chr8:73072906-73075498 bp, + strand Genetic Position: Chr8, 34.99 cM, cytoband C1
• Alliance: Klf2^tm1Hhn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228270
• Parent Cell Line: v6.4 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at a position on Chromosome 8, resulting in a 1594bp deletion starting at position 72319391 and ending at position 72320985, including exons 2 and 3 of the Klf2 gene. The targeting vector is composed of an FRT site followed by En2SA (mouse engrailed 2 gene splice acceptor), IRES-lacZ sequence, a loxP site, a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:228270)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klf2 Mutation: 12 strains or lines available

References

• Original: J:228270 Yeo JC, et al., Klf2 is an essential factor that sustains ground state pluripotency. Cell Stem Cell. 2014 Jun 5;14(6):864-72
• All: 1 reference(s)