Arhgap28^{tm2b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Arhgap28^{tm2b(KOMP)Wtsi}
• Name: Rho GTPase activating protein 28; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5750967
• Synonyms: Arhgap28^del, Arhgap28 del7- 9
• Gene: Arhgap28 Location: Chr17:68149708-68311115 bp, - strand Genetic Position: Chr17, 38.9 cM
• Alliance: Arhgap28^{tm2b(KOMP)Wtsi} page
• IMPC: Arhgap28 gene page

Mutation origin

• Mutant Cell Line: EPD0287_6_F11
• Germline Transmission: Earliest citation of germline transmission: J:222518
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 68178447 of Chromosome 17 upstream of the critical exon 7 through 9 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 through 9 at position 68180597. The critical exon 7 through 9 is thus flanked by loxP sites. Cre expression without flp expression, a reporter knockout mouse was created lacking the critical exons 7 through 9. RT-PCR confirmed the absence of full-length transcripts in pups. (J:222518)

Expression

In Mice Carrying this Mutation:

13 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arhgap28 Mutation: 50 strains or lines available

References

• Original: J:222518 Yeung CY, et al., Arhgap28 is a RhoGAP that inactivates RhoA and downregulates stress fibers. PLoS One. 2014;9(9):e107036
• All: 1 reference(s)