Mir193b^tm1.2Mam
Targeted Allele Detail

Summary

• Symbol: Mir193b^tm1.2Mam
• Name: microRNA 193b; targeted mutation 1.2, Lothar Hennighausen
• MGI ID: MGI:5750969
• Synonyms: miR-193b^f-
• Gene: Mir193b Location: Chr16:13267387-13267465 bp, + strand Genetic Position: Chr16, 9.29 cM
• Alliance: Mir193b^tm1.2Mam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241741
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to insert a single loxP site upstream, and a loxP and FRT site flanked neomycin resistance gene cassette downstream of the locus. The neo cassette was removed by flpe-mediated recombination. A null allele was created by removing the floxed locus through cre-mediated recombination. Absence of transcripts from this locus was confirmed by miR-seq and RNA-seq. (J:241741)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir193b Mutation: 2 strains or lines available

References

• Original: J:241741 Feuermann Y, et al., MiR-193b and miR-365-1 are not required for the development and function of brown fat in the mouse. RNA Biol. 2013 Dec;10(12):1807-14
• All: 2 reference(s)