Runx2^tm1Hkiy
Targeted Allele Detail

Summary

• Symbol: Runx2^tm1Hkiy
• Name: runt related transcription factor 2; targeted mutation 1, Hiroshi Kiyono
• MGI ID: MGI:5750983
• Synonyms: Runx2-I^neo
• Gene: Runx2 Location: Chr17:44806873-45125518 bp, - strand Genetic Position: Chr17, 21.33 cM
• Alliance: Runx2^tm1Hkiy page

Runx2^tm1Hkiy/Runx2⁺ and Runx2^tm1Hkiy/Runx2^tm1Hkiy mice show delayed bone ossification

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:223410
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Modified isoform(s)) Mutations: Insertion, Nucleotide substitutions Mutation details: The I isoform-specific translation initiation site in exon 2 was replaced with a stop codon (TGA) and a floxed neomycin resistance cassette inserted downstream of exon 2. RT-PCR analysis of E18.5 calvarial cells confirmed that expression of I isoform is severely reduced by 84% whereas expression of II isoforms is largely retained (>40%) in homozygous mutant mice. Total Runx2 expression is reduced by 67%. (J:223410)

Generation of the Runx2^tm1Hkiy allele

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx2 Mutation: 44 strains or lines available

References

• Original: J:223410 Okura H, et al., Runx2-I isoform contributes to fetal bone formation even in the absence of specific N-terminal amino acids. PLoS One. 2014;9(9):e108294
• All: 2 reference(s)