Cry2^tm1.1Yf
Targeted Allele Detail

Summary

• Symbol: Cry2^tm1.1Yf
• Name: cryptochrome circadian regulator 2; targeted mutation 1.1, Yoshitaka Fukada
• MGI ID: MGI:5751243
• Synonyms: CRY2(S557A)
• Gene: Cry2 Location: Chr2:92233991-92264388 bp, - strand Genetic Position: Chr2, 51.01 cM, cytoband E
• Alliance: Cry2^tm1.1Yf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224319
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 11 was replaced with an FRT- and loxP-flanked neomycin resistance cassette and modified exon 11 in which a point mutation (T to G) results in the amino acid substitution of alanine for serine at position 557 (S557A). Flp-mediated recombination removed the selection cassette. CRY2 protein expressed in the liver of homozygous mutant mice showed no immunoreactivity with the antibody recognizing Ser557-phosphorylated CRY2. (J:224319)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cry2 Mutation: 42 strains or lines available

References

• Original: J:224319 Hirano A, et al., In vivo role of phosphorylation of cryptochrome 2 in the mouse circadian clock. Mol Cell Biol. 2014 Dec;34(24):4464-73
• All: 1 reference(s)