Edn1^tm1Mki
Targeted Allele Detail

Summary

• Symbol: Edn1^tm1Mki
• Name: endothelin 1; targeted mutation 1, Masao Kakoki
• MGI ID: MGI:5751245
• Synonyms: L
• Gene: Edn1 Location: Chr13:42454952-42461466 bp, + strand Genetic Position: Chr13, 20.82 cM
• Alliance: Edn1^tm1Mki page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220962
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: A loxP site, a copy of the human AU/U rich elements of the human FOS gene, mouse Edn1 3' UTR, two copies of transcriptional insulators from the chicken ACTB gene, neo cassette, loxP site and Adm2 3' UTR were inserted just downstream of the stop codon via homologous recombination. In the absence of cre expression, the presence of the AU/U rich element destabilizes the mRNA, reducing expression. Following cre mediated recombination the Adm2 3' UTR stabilizes the mRNA, increasing expression. (J:220962)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Edn1 Mutation: 26 strains or lines available

References

• Original: J:220962 Hathaway CK, et al., Endothelin-1 critically influences cardiac function via superoxide-MMP9 cascade. Proc Natl Acad Sci U S A. 2015 Apr 21;112(16):5141-6
• All: 1 reference(s)