Akap11^tm1.2Jsco
Targeted Allele Detail

Summary

• Symbol: Akap11^tm1.2Jsco
• Name: A kinase anchor protein 11; targeted mutation 1.2, John D Scott
• MGI ID: MGI:5751858
• Synonyms: AKAP220-KO
• Gene: Akap11 Location: Chr14:78729686-78774248 bp, - strand Genetic Position: Chr14, 41.46 cM
• Alliance: Akap11^tm1.2Jsco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234905
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site and FRT-flanked neomycin cassette are introduced into intron 5 followed by a loxP site placed in intron 7. Flp-mediated recombination removed the FRT-flanked neo cassette. Cre-mediated recombination removed exons 6 and 7. Western blot anaylsis on nine tissues confirmed that no protein was expressed in mutant mice. (J:234905)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Akap11 Mutation: 65 strains or lines available

References

• Original: J:234905 Whiting JL, et al., AKAP220 manages apical actin networks that coordinate aquaporin-2 location and renal water reabsorption. Proc Natl Acad Sci U S A. 2016 Jul 26;113(30):E4328-37
• All: 4 reference(s)