Srrm4^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Srrm4^{tm1b(EUCOMM)Wtsi}
• Name: serine/arginine repetitive matrix 4; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5752029
• Synonyms: nSR100^delta7-8
• Gene: Srrm4 Location: Chr5:116577334-116729876 bp, - strand Genetic Position: Chr5, 56.45 cM, cytoband F
• Alliance: Srrm4^{tm1b(EUCOMM)Wtsi} page
• IMPC: Srrm4 gene page

Impaired neurite outgrowth of Srrm4^{tm1b(EUCOMM)Wtsi}/Srrm4^{tm1b(EUCOMM)Wtsi} motor neurons

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0538_3_A08, EPD0538_3_A09
• Germline Transmission: Earliest citation of germline transmission: J:221584
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 116605270 of Chromosome 5 upstream of exons 7 and 8 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 8 at position 116606407. Exons 7 and 8 are thus flanked by loxP sites. Cre mediated recombination without flp expression created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:221584)

Generation of the Srrm4^tm1b(EUCOMM)Wtsi allele

Expression

In Mice Carrying this Mutation:	40 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Srrm4 Mutation: 40 strains or lines available

References

• Original: J:221584 Quesnel-Vallieres M, et al., Essential roles for the splicing regulator nSR100/SRRM4 during nervous system development. Genes Dev. 2015 Apr 1;29(7):746-59
• All: 2 reference(s)