Ace2^em#Yngh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ace2^em#Yngh
• Name: angiotensin converting enzyme 2; endonuclease-mediated mutation, Huanming Yang
• MGI ID: MGI:5752578
• Synonyms: ACE2-KO
• Gene: Ace2 Location: ChrX:162922338-162971414 bp, + strand Genetic Position: ChrX, 76.12 cM, cytoband F5
• Alliance: Ace2^em#Yngh page

Mutation origin

• Strain of Origin: Kunming

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: TALEN technology was used to introduce mutations into exon 2. The population analyzed for phenotype contained individuals with alleles from 3 lines (#17, #20 and #24) containing: (1) a 4 bp deletion; (2) a 10 bp deletion; (3) a 3 bp deletion; (4) a 20 bp deletion with a single bp insertion; and (5) a single bp insertion. Northern blot analysis confirmed reduced expression of a long isoform. The short isoform is not detected in the colon of knock-out or control mice. The pound symbol (#) is used when no line is specified or lines are pooled. (J:221616)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace2 Mutation: 66 strains or lines available

References

• Original: J:221616 Liu C, et al., Generation of outbred Ace2 knockout mice by RNA transfection of TALENs displaying colitis reminiscent pathophysiology and inflammation. Transgenic Res. 2015 Jun;24(3):433-46
• All: 2 reference(s)