Dnajc7^tm1.2Neg
Targeted Allele Detail

Summary

• Symbol: Dnajc7^tm1.2Neg
• Name: DnaJ heat shock protein family (Hsp40) member C7; targeted mutation 1, Masahiko Negishi
• MGI ID: MGI:5752955
• Synonyms: CCRP deltaEx
• Gene: Dnajc7 Location: Chr11:100473644-100511014 bp, - strand Genetic Position: Chr11, 63.47 cM
• Alliance: Dnajc7^tm1.2Neg page

Steatotic liver and increased cholesterol levels in Dnajc7^tm1.2Neg/Dnajc7^tm1.2Neg males

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225381
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed the exons 2-4. Western blot analysis confirmed the absence of protein expression in nucleus and cytoplasm extracts of liver tissue. (J:225381)

Generation of the Dnajc7^tm1.1Neg and Dnajc7^tm1.2Neg alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnajc7 Mutation: 110 strains or lines available

References

• Original: J:225381 Ohno M, et al., The roles of co-chaperone CCRP/DNAJC7 in Cyp2b10 gene activation and steatosis development in mouse livers. PLoS One. 2014;9(12):e115663
• All: 1 reference(s)