Aspm^tm1(cre)Mrc
Targeted Allele Detail

Summary

• Symbol: Aspm^tm1(cre)Mrc
• Name: abnormal spindle microtubule assembly; targeted mutation 1, Mario R Capecchi
• MGI ID: MGI:5753079
• Synonyms: APSM^delta
• Gene: Aspm Location: Chr1:139382510-139421829 bp, + strand Genetic Position: Chr1, 61.45 cM, cytoband F
• Alliance: Aspm^tm1(cre)Mrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228026
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Aspm^tm1(cre)Mrc expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Aspm (MGI:1334448)

• Mutation details: The majority of exon 18 was replaced with an ires-cre cassette followed by a CAG-driven FRT-flanked neo gene via homologous recombination. The mutant protein lacks the terminal 1717 amino acids containing the calmodulin binding domain and the C-terminus. The deletion of a 2.6-kb genomic region of exon 18 yields a nonsense mutation resulting in a bicistronic mRNA product containing protein truncated at amino acid 1405, and ires-derived cre expression. Northern and Western blot analysis confirmed the predicted truncation of the mRNA and protein in homozygotes. (J:228026)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Aspm (mouse) Summary of all recombinase alleles driven by Aspm. Alliance mouse Aspm gene page

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aspm Mutation: 138 strains or lines available

References

• Original: J:228026 Capecchi MR, et al., ASPM regulates symmetric stem cell division by tuning Cyclin E ubiquitination. Nat Commun. 2015;6:8763
• All: 1 reference(s)