Gnao1^{tm2(EGFP/cre/ERT2)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gnao1^{tm2(EGFP/cre/ERT2)Wtsi}
• Name: guanine nucleotide binding protein, alpha O; targeted mutation 2, Wellcome Trust Sanger Institute
• MGI ID: MGI:5754964
• Gene: Gnao1 Location: Chr8:94537213-94696016 bp, + strand Genetic Position: Chr8, 45.94 cM
• Alliance: Gnao1^{tm2(EGFP/cre/ERT2)Wtsi} page

Mutation origin

• Mutant Cell Line: CEPD0102_3_H07
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMMTOOLS

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Gnao1^{tm2(EGFP/cre/ERT2)Wtsi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gnao1 (MGI:95775)

• Mutation details: According to the allele molecular image presented by the International Knockout Mouse Consortium, this allele has one loxP sites downstream of exon 3, and inserted into the intron between exons 2 and 3 is one FRT site, an engrailed 2 splice acceptor sequence, an EGFP and cre/ERT2 expression cassette, and a neomycin resistance cassette. (J:188991)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gnao1 (mouse) Summary of all recombinase alleles driven by Gnao1. Comparative matrix view of recombinase activities Alliance mouse Gnao1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnao1 Mutation: 29 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 1 reference(s)