Ino80^tm1.2Jland
Targeted Allele Detail

Summary

• Symbol: Ino80^tm1.2Jland
• Name: INO80 complex subunit; targeted mutation 1.2, Joseph Landry
• MGI ID: MGI:5755269
• Gene: Ino80 Location: Chr2:119203523-119308168 bp, - strand Genetic Position: Chr2, 59.97 cM, cytoband E5
• Alliance: Ino80^tm1.2Jland page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229516
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The targeting vector is designed to have a loxP site upstream of exon 2, and an FRT-flanked neo cassette followed by a second loxP site just downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette. Germ line, cre mediated recombination removed exons 2 through 4. Western blot analysis confirmed that no protein was expressed in mouse embryo fibroblasts. Two small transcripts are observed using RT-PCR. Sequencing identified these as aberrant exon 1-5 and exon 1-6 splice events. The first ATG of each splice product results in an out of frame transcript and a random protein product. (J:229516)

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ino80 Mutation: 440 strains or lines available

References

• Original: J:229516 Qiu Z, et al., Ino80 is essential for proximal-distal axis asymmetry in part by regulating Bmp4 expression. BMC Biol. 2016;14(1):18
• All: 1 reference(s)