Bwq14^NZO/HlLtJ
QTL Variant Detail

Summary

• QTL variant: Bwq14^NZO/HlLtJ
• Name: body weight QTL 14; NZO/HlLtJ
• MGI ID: MGI:5755429
• QTL: Bwq14 Location: unknown Genetic Position: Chr4, Syntenic

Variant origin

• Strain of Specimen: NZO/HlLtJ

Variant description

• Allele Type: QTL
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:172295

QTL Reference Notes

The Collaborative Cross (CC) is a large (~1,000 line) panel of recombinant inbred (RI) mouse strains being developed through a community effort (Churchill et al. 2004). The CC combines the genomes of eight genetically diverse founder strains - A/J, C57BL/6J, 129S1/SvImJ, NOD/ShiLtJ, NZO/HlLtJ, CAST/EiJ, PWK/PhJ, and WSB/EiJ - to capture nearly 90% of the known variation present in laboratory mice. CC strains are derived using a unique funnel breeding scheme. Once inbred, the RI CC lines can be used to generate thousands of potential 'outbred' but completely reproducible genomes through the generation of recombinant inbred crosses (RIX). The designation 'PreCC' is used to describe a mapping population of CC mice that is still at insipient stages of inbreeding. CTC (2004), Churchill, G. A., et al.. The Collaborative Cross, a community resource for the genetic analysis of complex traits. Nat Genet. 36, 1133-7.

Linkage analysis was performed on a mapping population of male PreCC#/Unc mice using 181,752 SNP markers to identify QTL associated with various exercise-related traits. PreCC#/Unc mice were derived from 176 lines lines ranging from generation G2:F5 to G2:F12. QTL were identified using a 1.5 LOD drop interval (genome build not given). Allele effect estimates suggest that WSB/EiJ alleles consistently contributed to increased wheel running distances at all QTL.

Five significant QTL were identified for four exercise-related traits:

QTL Bwq14 is significant for the trait "pre-exercise body weight" and maps to 3.32 - 10.34 Mb on Chromosome 4, with a peak LOD score of 7.9 at 7.54 Mb. C57BL/6J and NZO/HlLtJ alleles were reported to influence the Bwq14 locus. The protein coding gene Asph is a high-priority candidate gene for this locus.

QTL D12vesd is significant for the trait "running speed and distance, day 12." It maps to 32.54 - 38.34 Mb on Chromosome 16, with a peak LOD score of 7.13 at 33.25 Mb. A/J and WSB/EiJ alleles are reported to contribute to increased wheel running distance at the D12vesd locus.

QTL Fiq1 is significant for the trait "food intake during wheel running." It maps to 82.86 - 89.02 Mb on Chromosome 12, with a peak LOD score of 7.39 at 85.13 Mb. Allele effect estimates indicate that the C57BL/6J allele was the predominant contributor to increased food intake during wheel running at this locus.

QTL Weich7 is significant for the trait "post exercise net body weight" and maps to 72.74 - 83.45 Mb on Chromosome 6, with a peak LOD score of 7.23 at 77.72 Mb. Multiple progenitor alleles are reported to contribute to body weight at the Weich7 locus.

QTL Weich8 is significant for the trait "post exercise net body weight" and maps to 39.42 - 48.13 Mb on Chromosome 6, with a peak LOD score of 6.93 at 42.76 Mb. Multiple progenitor alleles are reported to contribute to body weight at the Weich7 locus. Multiple progenitor alleles are reported to contribute to body weight at the Weich8 locus.

PHENOTYPING DETAILS

Metabolism- and exercise-related traits were measured over a 3-wk period. Phenotypic measurements included body weight and body fat percentage before and after 12 days of wheel running, respiratory exchange ratio, food intake while sedentary or during wheel running, and running phenotypes such as speed and distance measured on days 1, 5, and 6, and 11 and 12.

During the 12 days of wheel running, food intake (FIRun) was measured every other day, and daily averages were calculated. Intakes were corrected for body weight (BW) by dividing average intakes by the average BW for the measurement period.

BW was measured at the beginning (BW) and end (BWpost) of the sedentary and wheel running phases of the experiment. Body composition was evaluated prior to the start [body fat percentage (BFP)] of wheel running and again after the 12-day wheel trial (BFpost) using MRI (EchoMRI, Houston, TX) to determine fat and lean mass percentages. Change in BW and BFP in response to exercise was calculated by subtracting the preexercise value from the postexercise value (BWnet and BFnet, respectively). Negative values indicate a loss of BW or fat in response to wheel running.

Wheel running was recorded continuously over a 12-day period using an automated activity wheel monitoring program (AWM; Lafayette Industries, Lafayette, IN). For data analysis, distance and speed data were directly extracted for day 1(D1, S1), and running distances and speeds were averaged for days 5 and 6 (D56, S56) and days 11 and 12 (D12, S12) of wheel access.

References

• Original: J:172295 Mathes WF, et al., Architecture of energy balance traits in emerging lines of the Collaborative Cross. Am J Physiol Endocrinol Metab. 2011 Jun;300(6):E1124-34
• All: 1 reference(s)