Col1a1^{tm20(tetO-GFP,-cas9*D10A)Slowe}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm20(tetO-GFP,-cas9*D10A)Slowe}
• Name: collagen, type I, alpha 1; targeted mutation 20, Scott Lowe
• MGI ID: MGI:5755843
• Synonyms: Apcⁿ, Col1a1^{tm20(RNU6-gRNA:Apc,tetO-GFP,-cas9*D10A)Slowe}
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm20(tetO-GFP,-cas9*D10A)Slowe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222595
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Endonuclease, Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: RMCE on KH2 cells inserted into the 3'UTR a RNU6 promoter driving expression of a paired guide RNAs targeting murine Apc 12 bp immediately upstream of the NGG protoscpacer-adjacent motif (PAM) sequence followed by a tetracycline response element promoter driving inducible expression of a GFP report gene, IRES sequence, human codon optimized CRISPR-associated endonuclease nickase variant cas9^D10A (Cas9n; derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) and polyadenylation sequence. (J:222595)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:222595 Dow LE, et al., Inducible in vivo genome editing with CRISPR-Cas9. Nat Biotechnol. 2015 Apr;33(4):390-4
• All: 1 reference(s)