Sco1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sco1^{tm1c(KOMP)Wtsi}
• Name: SCO1 cytochrome c oxidase assembly protein; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5758867
• Synonyms: Sco1^loxP
• Gene: Sco1 Location: Chr11:66943496-66957896 bp, + strand Genetic Position: Chr11, 40.59 cM
• Alliance: Sco1^{tm1c(KOMP)Wtsi} page
• IMPC: Sco1 gene page

Mutation origin

• Mutant Cell Line: EPD0125_1_E10
• Germline Transmission: Earliest citation of germline transmission: J:222036
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 66943973 of Chromosome 11 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 66945133. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. (J:222036)

Disease models

Expression

In Mice Carrying this Mutation:	94 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sco1 Mutation: 19 strains or lines available

References

• Original: J:222036 Hlynialuk CJ, et al., The Mitochondrial Metallochaperone SCO1 Is Required to Sustain Expression of the High-Affinity Copper Transporter CTR1 and Preserve Copper Homeostasis. Cell Rep. 2015 Feb 12
• All: 3 reference(s)