Csnk1a1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Csnk1a1^{tm1c(KOMP)Wtsi}
• Name: casein kinase 1, alpha 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5762595
• Gene: Csnk1a1 Location: Chr18:61688345-61723132 bp, + strand Genetic Position: Chr18, 34.62 cM, cytoband D3
• Alliance: Csnk1a1^{tm1c(KOMP)Wtsi} page
• IMPC: Csnk1a1 gene page

Mutation origin

• Mutant Cell Line: EPD0270_3_B02
• Germline Transmission: Earliest citation of germline transmission: J:216977
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 61700893 of Chromosome 18 upstream of the critical exon3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 61701699. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele which removes the neomycin/lacZ cassette. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:216977)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Csnk1a1 Mutation: 38 strains or lines available

Notes

Cell line EPD0125_5_H05 was successfully used to make chimeric mice. Germline transmission was accomplished. J:216977

References

• Original: J:216977 Schneider RK, et al., Role of casein kinase 1A1 in the biology and targeted therapy of del(5q) MDS. Cancer Cell. 2014 Oct 13;26(4):509-20
• All: 2 reference(s)