Gfer^tm1.1Crg
Targeted Allele Detail

Summary

• Symbol: Gfer^tm1.1Crg
• Name: growth factor, augmenter of liver regeneration; targeted mutation 1.1, Chandrashekhar R Gandhi
• MGI ID: MGI:5762603
• Synonyms: ALR^floxed
• Gene: Gfer Location: Chr17:24912164-24915065 bp, - strand Genetic Position: Chr17, 12.48 cM
• Alliance: Gfer^tm1.1Crg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227242
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette and left exons 2 and 3 floxed. (J:227242)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Gfer^tm1.1Crg/Gfer^tm1.1Crg	involves: 129 * C57BL/6
cn2	Gfer^tm1.1Crg/Gfer^tm1.1Crg Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: 129 * C57BL/6 * DBA

Phenotypes:

Affected Systems	cn1	cn2
show or hide all annotated terms
cellular	√	√
abnormal endoplasmic reticulum morphology		√
abnormal hepatocyte mitochondrial morphology		√
abnormal mitochondrial chromosome morphology	√
abnormal mitochondrial crista morphology		√
abnormal mitochondrial shape		√
decreased mitochondrial number		√
dilated mitochondrion		√
increased hepatocyte apoptosis		√
increased hepatocyte proliferation		√
abnormal cellular respiration		√
oxidative stress	√
growth/size/body		√
decreased body weight		√
increased liver weight		√
homeostasis/metabolism		√
increased circulating alanine transaminase level		√
decreased liver cholesterol level		√
increased liver cholesterol level		√
increased liver triglyceride level		√
immune system		√
liver inflammation		√
liver/biliary system	√	√
abnormal liver morphology		√
increased liver weight		√
decreased liver cholesterol level		√
increased liver cholesterol level		√
increased liver triglyceride level		√
abnormal hepatocyte morphology	√
abnormal hepatocyte mitochondrial morphology		√
hepatic necrosis		√
focal hepatic necrosis		√
hepatic steatosis		√
macrovesicular hepatic steatosis		√
microvesicular hepatic steatosis		√
increased hepatocellular carcinoma incidence		√
liver fibrosis		√
pale liver		√
abnormal liver physiology		√
increased hepatocyte apoptosis		√
increased hepatocyte proliferation		√
liver inflammation		√
abnormal oval cell physiology		√
neoplasm		√
increased hepatocellular carcinoma incidence		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gfer Mutation: 22 strains or lines available

References

• Original: J:227242 Gandhi CR, et al., Liver-specific deletion of augmenter of liver regeneration accelerates development of steatohepatitis and hepatocellular carcinoma in mice. Gastroenterology. 2015 Feb;148(2):379-391.e4
• All: 3 reference(s)