Mfsd14a^tm1Coll
Targeted Allele Detail

Summary

• Symbol: Mfsd14a^tm1Coll
• Name: major facilitator superfamily domain containing 14A; targeted mutation 1, William Colledge
• MGI ID: MGI:5763015
• Gene: Mfsd14a Location: Chr3:116424813-116456264 bp, - strand Genetic Position: Chr3, 50.42 cM, cytoband G2
• Alliance: Mfsd14a^tm1Coll page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:230570
• Parent Cell Line: CCB (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-lacZ reporter gene and neomycin resistance cassette replaced 70 bp in exon 4. The deletion results in a frame shift and premature termination at glycine 93. RT-PCR confirmed the absence of wild-type transcript. (J:230570)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mfsd14a Mutation: 85 strains or lines available

References

• Original: J:230570 Doran J, et al., Mfsd14a (Hiat1) gene disruption causes globozoospermia and infertility in male mice. Reproduction. 2016 Jul;152(1):91-9
• All: 1 reference(s)