Tg(Opalin-icre/ERT2)#Rjfl
Transgene Detail

Summary

• Symbol: Tg(Opalin-icre/ERT2)#Rjfl
• Name: transgene insertion, Robin JM Franklin
• MGI ID: MGI:5763107
• Synonyms: Opalin-iCreER^T2
• Transgene: Tg(Opalin-icre/ERT2)#Rjfl Location: unknown
• Alliance: Tg(Opalin-icre/ERT2)#Rjfl page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Opalin-icre/ERT2)#Rjfl expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Opalin (MGI:2657025)

• Mutation details: The mouse was generated via bacterial artificial chromosome transgenesis using the Opalin genomic bacterial artificial chromosome RP24-510M10. Insertion of the iCreERT2-polyA-frt-Kanamycin-frt cassette was achieved by homologous recombination (with no loss of the genomic bacterial artificial chromosome) in BAC (RPCI24-510M10) immediately after the Opalin initiation codon ATG of the open reading frame of the Opalin sequence. The frt-kanamycin-frt was removed using flippase recombinase. Four founders were generated, three of which expressed the transgene. The one with highest expression was chosen for further experimentation. Line numbers were not indicated. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:229987)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Opalin (mouse) Summary of all recombinase alleles driven by Opalin. Comparative matrix view of recombinase activities Alliance mouse Opalin gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:229987 Crawford AH, et al., Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination. Am J Pathol. 2016 Mar;186(3):511-6
• All: 3 reference(s)