Tg(RNU6-RNAi:Rhox3)2Wilk
Transgene Detail

Summary

• Symbol: Tg(RNU6-RNAi:Rhox3)2Wilk
• Name: transgene insertion 2, Miles F Wilkinson
• MGI ID: MGI:5763202
• Synonyms: Rhox3-shRNA
• Transgene: Tg(RNU6-RNAi:Rhox3)2Wilk Location: unknown
• Alliance: Tg(RNU6-RNAi:Rhox3)2Wilk page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Knockdown)
• Mutation: Insertion
• Tg(RNU6-RNAi:Rhox3)2Wilk involves 9 genes/genome features (Rhox3a, Rhox3a2, Rhox3b-ps ...) View all
• Mutation details: The transgene contains the human promoter, a floxed neomycin resistance gene/STOP cassette and a short hairpin RNA that targets all 8 Rhox3 paralogs (Rhox3a, Rhox3a2, Rhox3b-ps, Rhox3c, Rhox3d-ps, Rhox3e, Rhox3f, Rhox3g and Rhox3h). Lines 2, 11, 12, and 13 were generated with line 2 selected as the representative line. In addition to the reduced expression of targeted genes, mice exhibit reduced levels of all endogenous siRNAs tested. (J:229199)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:229199 Song HW, et al., shRNA off-target effects in vivo: impaired endogenous siRNA expression and spermatogenic defects. PLoS One. 2015;10(3):e0118549
• All: 1 reference(s)