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Sdf2l1em1(IMPC)J
Endonuclease-mediated Allele Detail
Summary
Symbol: Sdf2l1em1(IMPC)J
Name: stromal cell-derived factor 2-like 1; endonuclease-mediated mutation 1, Jackson
MGI ID: MGI:5766773
Gene: Sdf2l1  Location: Chr16:16948002-16950247 bp, - strand  Genetic Position: Chr16, 10.62 cM
Alliance: Sdf2l1em1(IMPC)J page
IMPC: Sdf2l1 gene page
Mutation
origin
Strain of Origin:  C57BL/6NJ
Project Collection: IMPC
Mutation
description
Allele Type:    Endonuclease-mediated (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThis allele from project Sdf2l1-7484J-F6196 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences CCCCGTTCCGAAACCCACAG, GGCAGGGACCCAAAGACAGA, GTGATTAACAAGAATCCACG and CTTTTTAGTGTTCAAGACGA, which resulted in a 60 bp deletion beginning at Chromosome 16 negative strand position 17,131,827 bp, AATCCACGGGGTGGGGTGGG, and ending after GCCAACAGTCGGTAACCGGC at 17,131,768 bp (GRCm38/mm10). This mutation deletes 26 bp of ENSMUSE00000130280 (exon 2) and 34 bp of intronic sequence immediately preceding the exon and removes the splice acceptor, in addition there is a 9 bp (ccactaaat) insertion at this site, as well as a 3 bp deletion in the intron 29 bp before the 60 bp deletion. Finally there is a 61 bp deletion 162 bp after the 60 bp deletion, which deletes the last 9 bp of the exon and will not alter the results of the mutation. This mutation is predicted to cause the loss of exon 2 and to result in a change of amino acid sequence after residue 63 and early truncation 8 amino acids later. (J:188991)
Inheritance:    Not Specified
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sdf2l1 Mutation:  15 strains or lines available
References
Original:  J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory