Sdf2l1em1(IMPC)J
Endonuclease-mediated Allele Detail
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Symbol: |
Sdf2l1em1(IMPC)J |
Name: |
stromal cell-derived factor 2-like 1; endonuclease-mediated mutation 1, Jackson |
MGI ID: |
MGI:5766773 |
Gene: |
Sdf2l1 Location: Chr16:16948002-16950247 bp, - strand Genetic Position: Chr16, 10.62 cM
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Alliance: |
Sdf2l1em1(IMPC)J page
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IMPC: |
Sdf2l1 gene page |
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Strain of Origin: |
C57BL/6NJ
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Project Collection: |
IMPC
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Allele Type: |
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Endonuclease-mediated (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: This allele from project Sdf2l1-7484J-F6196 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences CCCCGTTCCGAAACCCACAG, GGCAGGGACCCAAAGACAGA, GTGATTAACAAGAATCCACG and CTTTTTAGTGTTCAAGACGA, which resulted in a 60 bp deletion beginning at Chromosome 16 negative strand position 17,131,827 bp, AATCCACGGGGTGGGGTGGG, and ending after GCCAACAGTCGGTAACCGGC at 17,131,768 bp (GRCm38/mm10). This mutation deletes 26 bp of ENSMUSE00000130280 (exon 2) and 34 bp of intronic sequence immediately preceding the exon and removes the splice acceptor, in addition there is a 9 bp (ccactaaat) insertion at this site, as well as a 3 bp deletion in the intron 29 bp before the 60 bp deletion. Finally there is a 61 bp deletion 162 bp after the 60 bp deletion, which deletes the last 9 bp of the exon and will not alter the results of the mutation. This mutation is predicted to cause the loss of exon 2 and to result in a change of amino acid sequence after residue 63 and early truncation 8 amino acids later.
(J:188991)
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Inheritance: |
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Not Specified |
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012; |
All: |
3 reference(s) |
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