Med10^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Med10^em1(IMPC)J
• Name: mediator complex subunit 10; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:5766989
• Synonyms: Med10^em1J
• Gene: Med10 Location: Chr13:69950514-69964223 bp, + strand Genetic Position: Chr13, 35.55 cM
• Alliance: Med10^em1(IMPC)J page
• IMPC: Med10 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project Med10-7684J-M3179 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences TCCTGGACCAGGTCCTGTGA, CTCAAGCGTGTAAGCACACC, TGATGTCTGCACACGGACAA and GCTGAGTACTGCTACACTGT, which resulted in a 330 bp deletion spanning exon 3 beginning at Chromosome 13 positive strand position 69,813,580 bp, ACCCGGCTCCTGGACCAGGT, and ending after GGACTGCTGAGTACTGCTAC at 69,813,909 bp (GRCm38/mm10). This mutation deletes exon 3 and 227 bp of flanking intronic sequence including the splice acceptor and donor, and is predicted to cause a change of amino acid sequence after residue 69 and early truncation 5 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Med10 Mutation: 8 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)