Gpx4^tm4Marc
Targeted Allele Detail

Summary

• Symbol: Gpx4^tm4Marc
• Name: glutathione peroxidase 4; targeted mutation 4, Marcus Conrad
• MGI ID: MGI:5769979
• Synonyms: Gpx4_U46S
• Gene: Gpx4 Location: Chr10:79883000-79892273 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Gpx4^tm4Marc page

Gpx4^tm4Marc/Gpx4^tm4Marc embryos show resorptions and severe abnormalities of embryonic and extraembryonic structures

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225651
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

Allele Type: Targeted (Dominant negative) Mutations: Insertion, Nucleotide substitutions Mutation details: Exon 3 was replaced with a modified one in which two point mutations result in the amino acid substitution of a serine for selenocysteine at position 46 (U46S). A floxed neomycin resistance cassette was inserted downstream of exon 7. Immunoblot analysis revealed a significant up-regulation of Gpx4 protein levels in all somatic tissues of heterozygous mutant mice; however, significantly decreased levels of Gpx4-specific activities were detectable only in heterozygous livers, whereas no difference was seen in kidney, brain and heart. (J:225651)

Generation of the Gpx4^tm4Marc allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpx4 Mutation: 82 strains or lines available

References

• Original: J:225651 Ingold I, et al., Expression of a Catalytically Inactive Mutant Form of Glutathione Peroxidase 4 (Gpx4) Confers a Dominant-negative Effect in Male Fertility. J Biol Chem. 2015 Jun 5;290(23):14668-78
• All: 2 reference(s)