Prm2^em2Hsc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prm2^em2Hsc
• Name: protamine 2; endonuclease-mediated mutation 2, Hubert Schorle
• MGI ID: MGI:5770554
• Synonyms: Prm2delta¹⁰¹
• Gene: Prm2 Location: Chr16:10609241-10609969 bp, - strand Genetic Position: Chr16, 5.84 cM
• Alliance: Prm2^em2Hsc page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9-mediated genome editing generated a 101bp deletion in exon 1 leading to a frameshift that results in nonsense transcripts with a premature stop codon after amino acid 23. Western blot analysis of basic nuclear proteins isolated from epididymal sperm confirmed the absence of detectable mature protein in homozygous mutant males. (J:243626)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prm2 Mutation: 8 strains or lines available

References

• Original: J:243626 Schneider S, et al., Re-visiting the Protamine-2 locus: deletion, but not haploinsufficiency, renders male mice infertile. Sci Rep. 2016 Nov 11;6:36764
• All: 2 reference(s)