Mtxe4^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Mtxe4^C57BL/6J
• Name: methotrextate efficacy 4; C57BL/6J
• MGI ID: MGI:5770644
• QTL: Mtxe4 Location: unknown Genetic Position: Chr2, Syntenic

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:100362

In silico mapping was employed to identify loci associated with resistance to methotrextate (MTX) treatment in the murine air-pouch model of inflammation. Methotrextate significantly reduces inflammation (as measured by reduced leukocyte infiltration) and increases concentration of the anti-inflammatory agent adenosine in C57BL/6J and BALB/cJ, but not in DBA/1J or DBA/2J. A series of 5,268 SNPs spanning the mouse genome at 12 cM intervals was used for mapping analysis.

Mtxe1 (methotrextate efficacy 1)mapped to 8.7 cM - 21.1 cM and Mtxe2 (methotrextate efficacy 2) mapped to 40 cM - 87 cM on mouse Chromosome 1. These loci are linked to MTX anti-inflammatory efficacy and adenosine upregulation.

Mtxe3 (methotrextate efficacy 3) mapped to 12.9 cM - 49.8 cM and Mtxe4 (methotrextate efficacy 4) mapped to 60.1 cM - 94 cM on mouse Chromosome 2. These loci are linked to MTX anti-inflammatory efficacy and adenosine upregulation. Dpp4 (35 cM) and Fpgs (21.2 cM) are potential candidate genes for Mtxe3, and Ada (94 cM) is a potential candidate gene for Mtxe4.

Mtxe5 (methotrextate efficacy 5) mapped to 0 cM - 15.3 cM on mouse Chromosome 3. This locus is linked to MTX anti-inflammatory efficacy.

Mtxe6 (methotrextate efficacy 6) mapped to 0 cM - 21 cM and Mxte7 (methotrextate efficacy 7) mapped to 21 cM - 53 cM on mouse Chromosome 14. Mtxe6 is linked to MTX anti-inflammatory efficacy while Mtxe7 is linked to adenosine upregulation.

Mtxe8 (methotrextate efficacy 8) mapped to 0 cM - 17 cM on mouse Chromosome 15. This locus is linked to MTX anti-inflammatory efficacy.

Mtxe9 (methotrextate efficacy 9) mapped to 0 cM - 25 cM on mouse Chromosome 16. This locus is linked to adenosine upregulation.

References

• Original: J:100362 Delano DL, et al., Genetically based resistance to the antiinflammatory effects of methotrexate in the air-pouch model of acute inflammation. Arthritis Rheum. 2005 Aug;52(8):2567-75
• All: 1 reference(s)