Nat8l^tm1.2Meck
Targeted Allele Detail

Summary

• Symbol: Nat8l^tm1.2Meck
• Name: N-acetyltransferase 8-like; targeted mutation 1.2, Matthias Eckhardt
• MGI ID: MGI:5771691
• Gene: Nat8l Location: Chr5:34153328-34163260 bp, + strand Genetic Position: Chr5, 17.83 cM
• Alliance: Nat8l^tm1.2Meck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226682
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 was flanked by loxP sites and a loxP/FRT-flanked neomycin resistance gene followed by an IRES-lacZ cassette were inserted downstream of exon 3 via homologous recombination. The neomycin selection cassette was removed via Flp-mediated recombination and exon 3 was removed via cre-mediated recombination. Northern blot analysis confirmed the absence of RNA expression. (J:226682)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nat8l Mutation: 15 strains or lines available

References

• Original: J:226682 Maier H, et al., N-Acetylaspartate Synthase Deficiency Corrects the Myelin Phenotype in a Canavan Disease Mouse Model But Does Not Affect Survival Time. J Neurosci. 2015 Oct 28;35(43):14501-16
• All: 2 reference(s)