Gt(ROSA)26Sor^{tm3.2(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3.2(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3.2, Patricia Jensen
• MGI ID: MGI:5771695
• Synonyms: fsf-GFP, inter-hM3Dq-mCherry, RC::FL-hM3Dq, Rosa26^{CAG-fsf-eGFP-FLEX-hM3Dq-mCherry}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3.2(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235493
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The allele has a CAG hybrid promoter,a rox-flanked His3-SV40 transcriptional STOP cassette, an FRT-flanked STOP cassette and a variant lox-flanked region containing an eGFP cassette, an inverted hM3Dq/mCherry fusion protein, WPRE, polyA signal and an attB/attP-flanked PGK-frt-Neo-polyA cassette all inserted into the Gt(ROSA)26Sor locus. The inverted hM3Dq-mCherry-2ACT88 fusion protein cDNA sequence contains (hM3Dq DREADD) a red fluorescent protein mCherry and the final 264 coding nucleotides of the rat Htr2A gene (encoding a C-terminal epitope that mediates localization to the cell body/soma and dendrites). PhiC31-mediated recombination removed the AttB/AttP-flanked neo sequence. Dre-mediated recombination removed the rox-flanked STOP cassette. (J:235493)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:235493 Sciolino NR, et al., Recombinase-Dependent Mouse Lines for Chemogenetic Activation of Genetically Defined Cell Types. Cell Rep. 2016 Jun 14;15(11):2563-73
• All: 19 reference(s)