Exoc5^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Exoc5^{tm1c(KOMP)Mbp}
• Name: exocyst complex component 5; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:5774863
• Synonyms: Sec10^FL
• Gene: Exoc5 Location: Chr14:49249379-49304124 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Exoc5^{tm1c(KOMP)Mbp} page
• IMPC: Exoc5 gene page

Mutation origin

• Mutant Cell Line: DEPD00521_3_A02
• Germline Transmission: Earliest citation of germline transmission: J:226950
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 49273268 of Chromosome 14 upstream of the critical exons 7 through 10 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 7 through 10 at position 49275727. The critical exons 7 through 10 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:226950)

Disease models

Expression

In Mice Carrying this Mutation:	74 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Exoc5 Mutation: 36 strains or lines available

References

• Original: J:226950 Fogelgren B, et al., Urothelial Defects from Targeted Inactivation of Exocyst Sec10 in Mice Cause Ureteropelvic Junction Obstructions. PLoS One. 2015;10(6):e0129346
• All: 10 reference(s)