Strit1^em1Eno
Endonuclease-mediated Allele Detail

Summary

• Symbol: Strit1^em1Eno
• Name: small transmembrane regulator of ion transport 1; endonuclease-mediated mutation 1, Eric N Olson
• MGI ID: MGI:5774950
• Gene: Strit1 Location: Chr3:63388533-63391300 bp, - strand Genetic Position: Chr3, Syntenic
• Alliance: Strit1^em1Eno page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single CRISPR guide RNA was designed to target the coding sequence of exon 2 before the transmembrane region. This allele was generated by injecting the guide RNA and Cas9 mRNA into the pronucleus and cytoplasm and an allele containing a 2-bp insertion (GT into exon 2) that disrupts the ORF after codon 16 was chosen. This mutation is expected to produce a truncated protein lacking the transmembrane domain. (J:229305)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Strit1 Mutation: 0 strains or lines available

References

• Original: J:229305 Nelson BR, et al., A peptide encoded by a transcript annotated as long noncoding RNA enhances SERCA activity in muscle. Science. 2016 Jan 15;351(6270):271-5
• All: 2 reference(s)